Ashish Gupta | Department of Life Sciences

Profile

Ashish
Gupta
Assistant Professor
Department of Life Sciences
School of Natural Sciences (SoNS)
R208
Block-Research Block, Shiv Nadar University
Greater Noida
India
0120-3819100
Extn: 
246
Profile Summary 

Dr. Ashish Gupta did his doctorate from the prestigious Jawaharlal Nehru University, New Delhi where he worked in the area of molecular biology of malaria parasite. Thereafter, he moved to USA for his postdoctoral research in the lab of Prof. Anindya Dutta at the University of Virginia. During his post-doctoral, he worked on the area of mammalian biology, where the focus of his research was on functional analysis of chromatin modifier proteins and their modulation and function during viral infection. Currently his lab is interested in addressing several questions related to functional modulation of chromatin remodelling factors and their implicated under physiological and patho-physiological conditions. The findings of his research work are published in reputed international journals. He has been awarded with several prestigious national and international awards and fellowships.

Educational Qualifications 
B.Sc.
Bundelkhand University
M.Sc. (Biochemistry)
Jiwaji University, Gwalior
Ph.D.
Jawaharlal Nehru University, New Delhi
Work Experience 
06/13Present
Assistant Professor
Department of Life Sciences
Shiv Nadar University
I currently work here
09/09
12/12
Postdoctoral fellow
Department of Biochemistry and Molecular Genetics, School of Medicine
University of Virginia, USA
Teaching & Research Interests: 

Research Interest:

I. Chromatin Remodelling Factors & Nuclear Receptor Signalling

Since epigenetic regulators are critical for many basic biological processes, our lab is interested in elucidating the chromatin remodelling factors underlying the action of nuclear receptors and their implications in normal cellular physiology and pathophysiological conditions. One of our research focus is to understand how chromatin microenvironment and chromatin modifiers regulate nuclear receptor signalling and its functional dynamics.

TIP60, a histone acetyl transferase protein is known to regulate steroid receptor function in a context-dependent manner. Our lab has shown that TIP60 forms a novel complex with PXR, a xenobiotic receptor and together this complex promote cell migration and adhesion. Cell migration is fundamental to both normal cellular processes such as development, wound healing, tissue regeneration as well as in spread of metastatic cancers. We are currently studying the mechanism by which TIP60 and PXR work together to regulate the process of cell migration and adhesion and the physiological/pathophysiological implications resulting due to their aberrant activity.

II. Identification & characterization of novel molecular targets of human malaria parasite

Despite worldwide efforts ongoing to control and eliminate malaria, it still remains one of the deadliest parasitic infectious diseases that cause ~6.6 lakh deaths annually. Thus, there is an urgent need to identify and characterize novel molecular targets to deepen our understanding of the basic biology of malaria parasite and for strategizing better approach for anti-malarial therapy.

Given the essential role of epigenetics and chromatin remodelling factors in regulation of gene expression, the goal of our research project is to identify and characterize the molecular machinery involved in bringing epigenetic changes in the parasite genome during different stages of IDC. To gain insight into these mechanisms we are applying functional genomics approaches integrating molecular biology techniques, biochemistry and bioinformatics. To identify and characterize novel molecular targets for anti-malarial chemotherapy.

III. Host-Pathogen interaction

Infectious agents such as bacteria, virus or parasite when infect human host tries to alter the host genomic environment in such a manner that favours their survival and successive proliferation. An important strategy employed by these organisms to ensure their survival and replication is alteration of host epigenome leading to altered protein expression profile & inactivation/degradation of proteins related to cell-cycle check points and apoptotic pathways.

The major objective of this study is to investigate the role of pathogens in altering the host cellular protein expression profile and its role in pathogen survival and infection. Unravelling these molecular mechanisms is important to develop strategies to prevent infection of these pathogens.

 

Teaching Interests

PG Courses

1) Advance Molecular and Cell Biology (BIO-701)

2) Analytical Methodology (BIO-702)

UG Courses

1) Advances in Applied Biotechnology (CCC-409)

Conferences (Select): 

1. Invited speaker at ‘International Symposium on Malaria Biology’ and ‘29th National Congress of Parasitology on Basic and Applied Aspects’ held at University of Hyderabad, Hyderabad from 1st -3rd November 2018.

2. Invited speaker at ‘Rajasthan Science Congress’ held at Central University of Rajasthan, Ajmer from 13th -15th  October 2018.

3. Participated in ‘International Vaccine Conference & Malaria Workshop’ a conference cum workshop, organized by  the International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, held from 27th November-1st December 2017.

4. Invited speaker at National conference ‘Recent Trends in Cell and Molecular Biology Research’ held at Shiv Nadar University, Greater Noida on 27th and 28th October 2017.

5. Invited speaker for seminar series at Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, Kerala, held on 25th July 2017.

6. Invited speaker for the seminar series at the ‘Institute for Stem Cell Biology and Regenerative Medicine’ (inStem), Bangalore, held on 21st February 2017.

7. Invited speaker in “Group monitoring workshop” held by Science and Engineering Research Board (SERB), DST at Punjab University, Chandigarh, 16th & 17th June 2016.

8. Invited speaker in ‘Symposium on Current Trends in Drug Discovery Research in India’ held at Shiv Nadar University on 11th April 2015.

9. Participated in Sixth Symposium on ‘Frontiers in Molecular Medicine’ held at Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi held on 13-14th February 2015.

10. Invited speaker at 25th National conference on ‘Global Challenge in the Management of Parasitic Diseases: Host Parasite interactions, Molecular Biology and Drug Development’ by Indian Society for Parasitology at Central Drug Research Institute (CDRI), Lucknow from 16-18th October 2014.

11. Invited speaker at National conference on ‘Contemporary issues in Biotechnology: Progress & future applications.’ Amity University, Gurgaon held on 2nd April 2014.

12. Invited participant at Young Investigators Meeting (YIM) held at Hyderabad from 8-12th February 2014.

13. Invited participant at ‘National Cancer Institute Workshop on Virus and DNA damage’ held at Bethesda, Washington DC, USA from 11-12th September 2012.

14. Participated in conference ‘Antimicrobial drug resistance and the development of new antibiotics’ organized by Indian National Science Academy (INSA) & German Academy of Science Leopoldina, at INSA, New Delhi from 22-25th November 2007.

15. Participated in ‘International conference on malaria: Laveran to genomics’ Organised by Malaria research centre (ICMR), New Delhi, 4-6th November 2005.

Awards(Selected): 
2014

INSA Medal for Young Scientist-2015 by Indian National Science Academy, New Delhi. INSA medal is considered to be highest recognition of promise, creativity and excellence in a young scientist.

2013

Innovative Young Biotechnologist Award-2014 by the Department of Biotechnology (DBT), Ministry of Science & Technology, Government of India, New Delhi. IYBA award is given every year to outstanding young scientists in India to pursue innovative research in the frontier areas of biotechnology.

2013

Prof. M.B. Mirza Award-2014 by Indian Society for Parasitology. This award is given for best research work carried out in India in the field of parasitology.

2009

US Army Postdoctoral fellowship Award, Goverment of USA

2004

Council for Scientific and Industrial Research (CSIR), India ‘Senior Research Fellowship’

2002

Council for Scientific and Industrial Research (CSIR), India ‘Junior Research Fellowship’

2001

Awarded ‘Junior Research Fellowship’ from Indian Council for Medical research (ICMR), India

2001

Qualified Graduate Aptitude Test in Engineering (Life Sciences)

Scholarly Activity

Journal Publications (accepted or published)

  1. Sen U, Saxena H, Khurana J, Nayak A and Gupta A* (2018) Plasmodium falciparum RUVBL3 protein: a novel DNA modifying enzyme and an interacting partner of essential HAT protein MYST. Scientific Reports 8(1):10917 (*Corresponding author)
  2. Bhattacharyaa G, Girib RP, Dubey, Mitraa S, Priyadarshini R, Gupta A, Mukhopadhyay MK, Ghosha SK (2018) Structural changes in cellular membranes induced by ionic liquids: From model to bacterial membranes. Chemistry and Physics of Lipids 215: 1-10.
  3. Sharma R, Sharma B, Gupta A, Dhar SK (2018) Identification of a novel trafficking pathway exporting a replication protein, Orc2 to nucleus via classical secretory pathway in Plasmodium falciparum. Biochim Biophys Acta. 1865(5):817-829.
  4. Priti Kumari, Chintam Narayana, Shraddha Dubey, Ashish Gupta* and Ram Sagar* (2018) Stereoselective synthesis of natural product inspired carbohydrate fused pyrano [3,2-c]quinolones as antiproliferative agents. Organic and Biomolecular Chemistry DOI: 10.1039/C7OB03186F (*Corresponding author)
  5. Jaiswal B and Gupta A* (2018) Modulation of nuclear receptor function by chromatin modifying factor TIP60. Endocrinology 159(5):2199-2215. (*Corresponding author)
  6. Bakshi K, Ranjitha B, Dubey S, Jagannadham J, Jaiswal B, Gupta A* (2017) Novel complex of HAT protein TIP60 and nuclear receptor PXR promotes cell migration and adhesion. Scientific Reports 7(1):3635. (*Corresponding author)
  7. Bhattacharya G, Giri RP, Saxena H, Agrawal VV, Gupta A, Mukhopadhyay MK, Ghosh SK (2017) X-ray Reflectivity Study of the Interaction of an Imidazolium-Based Ionic Liquid with a Soft Supported Lipid Membrane. Langmuir 33(5):1295-1304.
  8. Deshmukh AS, Agarwal M, Mehra P, Gupta A, Gupta N, Doerig CD and Dhar SK (2015) Regulation of Plasmodium falciparum Origin Recognition Complex subunit 1 (PfORC1) function through phosphorylation mediated by CDK-like kinase PK5 Molecular Microbiology 98(1):17-33.
  9. Jha S*, Gupta A*, Dar A and Dutta A (2013) RVBs are required for assembling a functional TIP60 complex. Molecular and Cellular Biology 33(6):1164-74. * Co first author
  10. Gupta A, Jha S, Engel D, Ornelles D and Dutta A (2013) Tip60 Degradation by Adenovirus Relieves Transcriptional Repression of Viral Transcriptional Activator EIA. Oncogene 32(42):5017-25.
  11. Deshmukh AS, Srivastava S, Hermann S, Gupta A, Mitra P, Gilberger TW and Dhar SK (2012) The role of N-terminus of Plasmodium falciparum ORC1 in telomeric localization and var gene silencing. Nucleic Acids Research 40: 5313–533.
  12. Gupta A, Mehra P, Deshmukh A, Dar A, Mitra P, Roy N., and Dhar S.K. (2009) Functional dissection of the catalytic carboxyl- terminal domain of origin recognition complex subunit1 (PfORC1) of the human malaria parasite Plasmodium falciparum. Eukaryotic Cell 8: 1341-1351.
  13. Gupta A, Mehra P, and Dhar S.K. (2008) Plasmodium falciparum origin recognition complex subunit 5: functional characterization and role in DNA replication foci formation. Molecular Microbiology 69: 646- 665. (Highlighted by Faculty of 1000)
  14. Prusty D, Mehra P, Srivastava S, Shivange A.V., Gupta A, Roy N, and Dhar, S.K. (2008) Nicotinamide inhibits Plasmodium falciparum Sir2 activity in vitro and parasite growth. FEMS Microbiology Letters 282: 266-72.
  15. Gupta A, Mehra P, Nitharwal R, Sharma A, Biswas AK and Dhar, S.K. (2006) Analogus expression pattern of Plasmodium falciparum replication initiation proteins PfMCM4 and PfORC1 during the asexual and sexual stages of intraerythrocytic developmental cycle. FEMS Microbiology Letters 261: 12-18.
  16. Mehra P, Biswas AK, Gupta A, Gaurinath S, Chitnis C and Dhar S.K. (2005) Expression and characterization of human malaria parasite Plasmodium falciparum origin recognition complex subunit1. Biochemical Biophysical Research Communication 337: 955-966.
  1. Shymal K Goswami, Pramod C. Rath, H.K. Das, Suman K. Dhar, Ashish Gupta. ‘Molecular Biology’ in Text Book of Biotechnology (5th Edition) by Wiley India (2017) ISBN: 9788126564040.
  2. Bharti Jaiswal and Ashish Gupta*. ‘Developments in Medical Biotechnology: Present and Challenges’; Biotechnology - Progress and Prospects, Studium Press LLC, Texas, US. (2015) ISBN: 1-62699-059-X and ISBN: 978-162699-059-3 (*Corresponding author)
  3. Ashish Gupta, Parul Mehra and Suman K. Dhar. ‘Plasmodium falciparum Origin Recognition Complex (ORC): A master regulator of parasite DNA replication and virulence gene expression’. Proceedings of IISc Centenary conference – Mechanisms of Microbial Pathogenesis, Indian Institute of Science, Bangalore, India (2009).