Dr. Manasi Mishra has been working as DST-INSPIRE Faculty at Department of Life Sciences, Shiv Nadar University, Gautam Buddha Nagar, UP. She received her Ph.D in Biotechnology from CSIR-National Chemical Laboratory, Pune. She is also a recipient of short-term DAAD fellowship and conducted collaborative research at EMBL Hamburg, Germany. After completing her doctoral research, she worked at the Institute of Organic Chemistry and Bio-Chemistry, Academy of Sciences of the Czech Republic, Prague.
Plant immunity proteins and peptides, bioactive peptides and their therapeutic applications
She has been actively working in the area of plant immunity proteins and peptides involved in defense against insect pest infestation and those with relevance in the context of crop improvement. Her research has been focused on structure-function correlation of diverse plant defense proteins, molecular diversity and their functional significance. She is also working extensively towards understanding the cyclic peptides distribution within individual plants and their tissues, factors influencing cyclotide expression, gene architecture, post-translational mechanism leading to cyclization, bioactivities and designing synthetic cyclotides directed towards molecular targets in human parasitic diseases like malaria and leishmania. She has published her research in international peer-reviewed journals and presented in international conferences.
Research Interests: Plant immunity proteins and peptides, Plant bioactive molecules, Botanical therapeutics, Molecular diversity and evolution
Teaching Interests: Plant physiology and biochemistry
- Participation in ICGEB workshop 2018 “Smart metabolic engineering of plants for drugs biosynthesis” held at ICGEB, New Delhi, India 16-17 March, 2018.
- Oral presentation: Mishra M (2017) “Cyclotides: A plethora of plant defense peptides against biotic stress” In: Agrigenomics India 2017, 3rd International conference on Plant Genetics & Genomics during July 20-21, 2017 in Chandigarh, India
- Poster presentation: Mishra M, Brynda J, Mares M (2015) In: 29th Annual Symposium of Protein Society, Barcelona, Spain during 22-25 July, 2015.
- Poster presentation: Mishra M, Tamhane VA, Khandelwal N, Giri AP, Kulkarni MJ and Gupta VS (2011) In: Sixth International Symposium on Molecular Insect Science held at NH Grand Krasnapolsky, Amsterdam, The Netherlands during 2-5 October, 2011.
- Poster presentation: Mishra M, Tamhane VA, Khandelwal N, Giri AP, Kulkarni MJ and Gupta VS (2009) In: The Sixth Solanaceae Genome Workshop held at Hotel Le Meridien, New Delhi, India during November 8-13, 2009.
- Indo-Australian Career Boosting Gold Fellowships (IACBGF-2018) from Department of Biotechnology, Government of India [Visiting Research Fellow- La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia]
- DST-INSPIRE Faculty Award (Plant Sciences & Agriculture) July session 2015 from Department of Science & Technology, Government of India.
- Travel award from ‘THE PROTEIN SOCIETY’ for participation in 29th Annual Symposium of Protein Society, Barcelona, Spain (22nd to 25th July, 2015).
- Travel Award (ITS) from Department of science and technology (DST), Govt. of India for participation in International conference on Molecular insect science in Amsterdam, Netherlands (2nd to 5th October, 2011).
- ‘Short-Term Scholarship’ (June-November 2010) from DAAD. Work Place: European Molecular Biology Laboratory (EMBL), Hamburg, Germany.
- Best Poster Award at National Science Day, 2010 at the National Chemical Laboratory, Pune.
- Best Poster Award at National Science Day, 2009 at the National Chemical Laboratory, Pune.
- National Eligibility Test (NET, JRF 2006) conducted by Council of Scientific and Industrial Research and University Grant Commission (CSIR-UGC), Government of India.
- Mishra M, Tamhane VA, Khandelwal N, Kulkarni MJ, Gupta VS and Giri AP (2010) Interaction of recombinant CanPIs with Helicoverpa armigera gut proteases reveals their processing patterns, stability and efficiency. Proteomics 10(15):2845-2857.
- Mishra M, Mahajan N, Tamhane VA, Kulkarni MJ, Baldwin IT, Gupta VS and Giri AP (2012) Stress inducible proteinase inhibitor diversity in Capsicum annuum. BMC Plant Biology12:217. [Highly Accessed]
- Mishra M, Joshi RS, Gaikwad S, Gupta VS and Giri AP (2013) Structural-functional insights of single and multi-domain Capsicum annuum protease inhibitors. Biochemical and Biophysical Research Communications 430(3): 1060-1065.
- Joshi RS, Mishra M, Tamhane VA, Ghosh A, Sonavane U, Suresh CG, Joshi R, Gupta VS and Giri AP (2014)The remarkable efficiency of a Pin-II proteinase inhibitor sans two conserved disulfide bonds is due to enhanced flexibility and hydrogen-bond density in the reactive site loop. Journal of Biomolecular Structure and Dynamics 32 (1): 13-26.
- Mahajan N, Mishra M, Tamhane VA, Gupta VS and Giri AP (2013) Plasticity of protease gene expression in Helicoverpa armigera upon exposure to multi-domain Capsicum annuum protease inhibitor.Biochim et Biophys Acta: General subjects 1830(6):3414-3420.
- Mahajan N, Mishra M, Tamhane VA, Gupta VS and Giri AP (2014) Stress inducible proteomic changes in Capsicum annuum leaves. Plant Physiology and Biochemistry 74:212-217.
- Mishra M, Lomate PR, Joshi RS, Punekar SA, Gupta VS and Giri AP (2015). Ecological turmoil in evolutionary dynamics of plant-insect interactions: defense to offence. Planta 242(4): 761-71 (DOI 10.1007/s00425-015-2364-7).
- Joshi RS, Mishra M, Suresh CG, Gupta VS and Giri AP (2013). Complementation of intramolecular interactions for structural-functional stability of plant serine proteinase inhibitors. Biochim et Biophys Acta: General subjects 1830: 5087-5094.
- Mishra M, Singh V and Singh S (2019) Review: Structural Insights Into Key Plasmodium Proteases as Therapeutic Drug Targets. Front. Microbiol. 10:394. [doi: 10.3389/fmicb.2019.00394] [IF: 4.0]
- Mishra M* (2020) Evolutionary aspects of the structural convergence and functional diversification of Kunitz-domain inhibitors. Journal of Molecular Evolution 88(7): 537-548. Springer (https://doi.org/10.1007/s00239-020-09959-9). Read-only view of the full article accessible at: https://rdcu.be/b5Lxt
- Mishra M#, Singh V#, Tellis MB, Joshi RS, Pandey KC, Singh S (2020) Cyclic peptide engineered from phytocystatin inhibitory hairpin loop as an effective modulator of falcipains and potent anti-malarial. Journal of Biomolecular Structure and Dynamics (https://doi.org/10.1080/07391102.2020.1848629). [# Equal contribution]. Eprint link: https://www.tandfonline.com/eprint/WU6AWRSGFDMR6ICSWVHH/full?target=10.1080/07391102.2020.1848629
- Mishra M*, Singh V, Tellis MB, Joshi RS, Singh S* (2021) Repurposing the McoTI-II rigid molecular scaffold in to inhibitor of ‘papain superfamily’ cysteine proteases. Pharmaceuticals 14, 7: 1-17. MDPI (https://doi.org/10.3390/ph14010007).